Xibei Zhiwu Xuebao 27 (3): 460-463.Ĭho JS, Han JH, Lee CH. Gametophyte development and its diversity in Cibotium barometz. anthriscifolia in vitro culture: sporophyte development and volatile compound profile of an aromatic fern. Amer Fern J 83 (3): 79-85.Ĭastilho CVV, Neto JFF, Leitão SG, Barreto CS, Pinto SC, da Silva NCB. Spore Germination and Early Gametophyte Development of Platycerium bifurcatum. Plant Cell Tissue & Organ Cult 57: 65-69.Ĭamloh H. In vitro organogenesis of Polypodium cambricum. īertrand AM, Albuerne MA, Fernandez H, Gonzalez A, Sanchez-Tames R. Fern conservation: Spore, gametophyte, and sporophyte ex situ storage, in vitro culture, and cryopreservation. Drug Design, Develop & Therapy 11: 995–1009. The antiulcer effect of Cibotium barometz leaves in rats with experimentally induced acute gastric ulcer. Īl-Wajeeh NS, Hajrezaie M, Al-Henhena N, Kamran S, Bagheri, E, Zahedifard M, Abdulla MA. The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats. ĪL-Wajeeh NS, Hajerezaie M, Noor SM, Halabi MF, Al-Henhena N, Azizan AHS, Abdulla M A. Indirect organogenesis in milkweed (Calotropis procera) from mature zygotic embryo explants. barometz gametophytes were ½ MS with or without 2 mg L -1 BAP. The two best spore culture media for rapid spore germination and development of C. Young heart-shape gametophytes consisting of 110-240 cells were formed in 45-61 days after sowing. Spore germination started 7-14 days after sowing. barometz was observed to be Vittaria-type, and its prothallial development was Drynaria-type. A factorial combination of four BAP concentrations (0, 2, 4, and 6 mg L -1) with four concentrations of NAA (0 0.01 0.03 and 0.05 mg L -1) created 16 treatments replicated in a Completely Randomized Design. The sterilized spores were sown in half-strength Murashige & Skoog (½MS) basal medium supplemented with combinations of 6-Benzylaminopurine (BAP) and ?-Naphthalene acetic acid (NAA). barometz, and (ii) to determine the best culture medium for rapid spore germination and early development of the gametophytes. This study’s objectives were: (i) to observe in vitro spore germination and early gametophyte development of C. In vitro culture is one of the technologies used for ex-situ propagation and conservation of rare and endangered ferns and lycophytes. Globally, populations of this species are under significant pressure due to overexploitation in the wild. It is an important export commodity for traditional and modern medicine. is known as the golden chicken fern and included in Appendix II of CITES. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J.